Program

Introduction by Session Chair: David Tollervey (The University of Edinburgh)

> Detecting and characterizing the first snoRNAs
David Tollervey (The University of Edinburgh)

> Toward Reliable snoRNA Quantification: Benchmarking Reverse Transcriptases and Methodological Controls
Sherif Abou Elela (Université de Sherbrooke)

> snoRNAs or snoRTs: how can we accurately quantify them?
Giulia Venturi (Alma Mater Studiorum - Università di Bologna)

> Expanding the Capabilities of snoRNA Detection and Modification Profiling with NERD-seq
Athanasios Zovoilis (University of Manitoba)

> Panel Discussion and Conclusion

Introduction by Session Chair: Blake Sweeney (EMBL-EBI)

> Nomenclature issues from the perspective of RNAcentral and Rfam
Blake Sweeney (EMBL-EBI)

> Comprehensive Annotation and Expression Dynamics of the Zebrafish snoRNAome
Renata Hamar (Travel Award Winner, Eötvös Loránd University)

> Annotating snoRNAs in GENCODE
Zoe Hollis (EMBL-EBI)

> Nomenclature and annotations to facilitate the study of snoRNAs eukaryote-wide
Michelle Scott (Université de Sherbrooke)

> Identification of rRNA modifications and their corresponding snoRNAs in Drosophila melanogaster
Jean-Yves Roignant (The University of Lausanne)

> Panel Discussion and Conclusion

Introduction by Session Chair: Carsten Müller-Tidow (Heidelberg University Hospital)

> Assigning functions to ribosomal 2’-O-methylations
Anders Lund (The University of Copenhagen)

> An Unexpectedly Large Repertoire of Box C/D Small RNAs Revealed in Mouse Tissues
Jérôme Cavaillé (LBME-CNRS)

> Decoding specialized ribosomes: challenges in functional validation of rRNA modifications
Mie Monti (Travel Award Winner, Centre for Genomic Regulation)

> Panel Discussion and Conclusion

1. snoRNA Detection Workshop and Development of Recommendation
(Elela, Tollervey)
2. snoRNA Nomenclature and Cross-Species Comparison Workshop
(Scott, Sweeney)
3. Standardization of Methods for Studying Functions of snoRNA
(Carsten Muller-Tidow, Ruggero)

Trainees present their posters on research related to snoRNA and ribosomal RNA modifications This session provides an opportunity for feedback and discussion with senior researchers and peers.

Introduction by Session Chair: Denis L.J. Lafontaine (Vrije Universiteit Brussel)

> First generation sequencing-based methods for RNA-guided rRNA modifications
Henrik Nielsen (The University of Copenhagen)

> LC-MS based quantification of modified ribonucleosides: an effort towards global harmonization of protocols and standards
Mark Helm (Johannes Gutenberg University of Mainz)

> Decoding the rRNA modification landscape using native RNA nanopore sequencing
Eva Novoa (Centre for Genomic Regulation)

> Sci-ModoM: roadmap to facilitate access to and sharing of high-throughput transcriptome-wide RNA modification data, to harmonize data exchange formats and interoperability standards
Etienne Boileau (Heidelberg University Hospital)

> Computational limitations and future needs to unravel the full potential of 2'-O-Methylation and C/D Box snoRNAs
Christian Ramirez (Travel Award Winner, Università degli Studi di Trento)

> Panel Discussion and Conclusion

Introduction by Session Chair: Marianna Penzo (Alma Mater Studiorum - Università di Bologna)

> Cell-free translation assays: corner-stone approaches to explore ribosome-based translation regulation
Marianna Penzo (Alma Mater Studiorum - Università di Bologna)

> rRNA Chemical Modifications as Dynamic Translational Regulators in Cancer and Chemoresistance
Jean-Jacques Diaz (Université Claude Bernard Lyon 1)

> Workflows and methods for assessing impact of dynamic rRNA 2’-O-methylations on translation and cell phenotypes
Martin Jansson (The University of Copenhagen)

> Challenges in Linking snoRNA-Guided rRNA Modifications to Translation Regulation
Laurence Faucher-Giguere (Université de Sherbrooke)

> Uncovering the impact of snoRNA-guided rRNA modification heterogeneity in a model of non-small cell lung cancer invasion
Sarah Webster (Travel Award Winner, Emory University)

> Panel Discussion and Conclusion

Introduction by Session Chair: Alan Warren (University of Cambridge)

> Discriminating Between Catalytic and Non-Catalytic Functions of snoRNPs
Brigitte Pertschy (University of Graz)

> RNA binding by H/ACA snoRNPs – near-cognate interactions beyond pseudouridine formation
Ute Kothe (University of Manitoba)

> H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation
Matthias Thoms (Ludwig Maximilian University of Munich)

> Mapping RBP-specific snoRNA interactions with chimeric eCLIP
Eric Van Nostrand (Baylor University)

> Panel Discussion and Conclusion

1. Development of Methods for Standardizing Modification Detection and Quantification Methods
(Bohnsack, Marcel, Lafontaine)

2. Tools and Approaches for Systematic Identification of snoRNAs for rRNA Modifications
(Ghalei, Lafonfaine, Warren)

3. Standardization of Inhibiting and Studying the Effect of Ribosomal rRNA Modification
(Montanaro, Penzo)

Break away time to assemble conclusions (Co-organizers, Chairs, Moderators)
Let’s meet the Editors (Co-organizers and Editors)

(poster awards, etc.)

Publishing a Guideline for the Study of Ribosomal RNA Modifications and snoRNAs
Meeting Concludes